Fig. 1: Sulfatase is expressed by mouse and human OPCs. | Nature Communications

Fig. 1: Sulfatase is expressed by mouse and human OPCs.

From: Overcoming the inhibitory microenvironment surrounding oligodendrocyte progenitor cells following experimental demyelination

Fig. 1

a RT-PCR analysis of SULF2 mRNA in human primary OPCs during in vitro differentiation with and without PDGF-AA removal (no GF) (mean ± SEM, n = 4 fetal samples). b, c, SULF2 protein (green) is expressed at low levels in hOPCs (b), and immunoreactivity substantially increased after treatment with the protein secretion inhibitor brefeldin A (5 µg/ml) (c). Confocal micrograph of SULF2 mRNA (green)-expressing PDGFRA+ OPCs (red) in fetal human brain (d). e–g Sulf1 and Sulf2 expression was analyzed by confocal microscopy in mouse corpus callosum during normal development by RNAscope in situ hybridization and combined with Olig2 immunohistochemistry (IHC) at postnatal day 7 (e, h), day 28 (f, i) and at 24 weeks (g, j). DAPI (blue), Sulf1 mRNA (gray), Sulf2 mRNA (green), Pdgfra mRNA (red), and Olig2 protein (cyan). White arrows denote Pdgfra+ OPCs expressing Sulf1 and Sulf2. Scale: 100 µm (b, c), 20 µm (d), and 20 µm (e–j).

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