Fig. 3: Electrical measurements on NupX-coated solid-state nanopores.
From: A designer FG-Nup that reconstitutes the selective transport barrier of the nuclear pore complex
a, b Schematic of the nanopore system before (a) and after (b) NupX functionalization. c, d Examples of raw current traces through bare (c) and NupX-coated (d) pores, recorded under 100 mV applied bias for different analyte conditions. Current traces are recorded in the presence of buffer only (top), upon addition of 450 nM Kap95 (middle) and 2.8 μM BSA (bottom). Traces were filtered at 5 kHz. e Scatter plot showing conductance blockades and dwell time distributions of translocation events of the analytes Kap95 (black, N = 506) and BSA (red, N = 387) through a bare 30 nm pore, recorded over the same time interval. f Scatter plot showing conductance blockades and dwell time distributions of translocation events of the analytes Kap95 (yellow, N = 686) and BSA (blue, N = 28) through a NupX-coated 30 nm pore, recorded over the same time interval. Top and right panels in e and f show lognormal fits to the distribution of dwell times and conductance blockades, respectively. Dashed vertical lines in top panels indicate the mean values for the dwell time distributions. g Average event rate of translocations for Kap95 through a bare pore (black), BSA through a bare pore (red), Kap95 through a NupX-coated pore (yellow) and BSA through a NupX-coated pore (blue). Error bars indicate standard deviations from independent measurements (circles) on three different pores, N = 3.
