Fig. 3: Single-cell transcriptome analysis and IGH lineage analysis of B cells from TNBC patients at low resolution. | Nature Communications

Fig. 3: Single-cell transcriptome analysis and IGH lineage analysis of B cells from TNBC patients at low resolution.

From: Atlas of breast cancer infiltrated B-lymphocytes revealed by paired single-cell RNA-sequencing and antigen receptor profiling

Fig. 3

a The t-SNE projection of 8632 B cells from TNBC patients shows four major cellular clusters. b Selected marker genes to define each B cell cluster. c The distributions of four clusters in each patient sample. The p values were calculated by two-tailed paired Student’s t test. d Heat maps show the distribution of the same IGH germline events (upper panel) and the same IGH sequence events (lower panel) among the four B cell clusters for TNBC and PBMC samples. The same germline events were calculated using clonally related B cells, and the same IGH sequence events were calculated using B cells with the same observed IGH sequences. Then, the event numbers were normalized by the cell numbers of related B cell clusters and plotted in the heatmap. See “Paired BCR and single-cell RNA-seq data analyses” in the “Methods” section for details. Source data are provided as a source data file.

Back to article page