Fig. 4: Single-cell transcriptome analysis and IGH lineage analysis of B cells from TNBC patients at a higher resolution. | Nature Communications

Fig. 4: Single-cell transcriptome analysis and IGH lineage analysis of B cells from TNBC patients at a higher resolution.

From: Atlas of breast cancer infiltrated B-lymphocytes revealed by paired single-cell RNA-sequencing and antigen receptor profiling

Fig. 4

a The t-SNE projection of 8632 B cells from TNBC patients shows 13 major cellular clusters at a higher resolution. b Selected marker genes to define each B cell cluster. c The distributions of 13 clusters in each patient sample (upper panel: PBMC; lower panel: TNBC). X represents B cell clusters, and Y represents the cell percentage in each sample. Each dot represents one sample, and error bars represent the mean ± standard deviation (SD) of the five samples. d Heat maps show the distribution of the same germline event number (upper panel) and the same IGH sequence event number (lower panel) among the 13 B cell clusters for TNBC and PBMC samples. The same germline events were calculated using clonally related B cells, and the same V(D)J sequence events were calculated using B cells with the same observed IGH sequences. Then, the event numbers were normalized by the cell numbers of related B cell clusters and plotted in the heatmap. See “Paired BCR and single-cell RNA-seq data analyses” in the Methods section for details. Source data are provided as a source data file.

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