Fig. 2: Primary cultured SIRPα deficient microglia engulf more synaptic elements.
From: Loss of microglial SIRPα promotes synaptic pruning in preclinical models of neurodegeneration
a Diagrams show neuron-microglia co-cultures (a′ and a″) and Sholl analyses (a″′) that quantifies synaptic puncta around microglia. Green cells in diagrams represent neurons (MAP2+), red puncta represent synaptic marker (SPH/PSD95), and blue cells represent microglia (Iba-1+). b–d Confocal images (b) show the presence of synaptic puncta (SPH+/PSD95+) around SIRPα+/+ or SIRPα−/− microglia (Iba1+). Scale bar, 50 μm. Statistical analysis for Syanptophysin (c) or PSD95 density (d) is obtained by two-way ANOVA; n = 5 independent experiments. e–g 3D reconstruction and surface rendering demonstrate that SIRPα−/− microglia engulf more synaptic elements. Grid line increments = 5 μm. Histograms depict the statistics of synaptic engulfment. Two-tailed unpaired t-test, n = 5 independent experiments, average of 3–4 microglia from each experiment. SPH synaptophysin, MG microglia. Data are mean ± s.e.m. ***P < 0.001. Detailed statistical information was listed in Supplementary Statistical Data. Source data are provided as a Source Data file.
