Fig. 4: Dystrophin isoforms expression in thymus of 3-month-old C57Bl and mdx mice.

Confocal microscope fluorescence images (right) and tile scan reconstructions (left) of dystrophin isoforms in thymi of C57Bl and mdx mice. DYS-1 (mid-rod-domain) (red) and DYS-2 (C-terminal-domain) (red) antibodies were used (a). RT-PCR analysis of the thymus of C57Bl mice determined the expression of Dp71, Dp427 dystrophin isoforms (b). Following the purification of the PCR products related to the isoforms Dp260, Dp140, and Dp116, we performed another PCR on these samples and we found one band corresponding to Dp140 in C57Bl thymus (c). RT-PCR analysis of thymus of mdx mice determined the expression of the only Dp71 dystrophin isoform (d). WB analysis confirmed the absence of Dp427 dystrophin isoform in thymus of mdx mice and the presence of DP71 dystrophin isoform in thymus of both C57Bl and mdx mice (e). Densitometric analyses are shown as DP71/β-Tubulin III (e). Scale bar: 100 μm for tile scan reconstructions and 50 μm for fluorescence images (a). MW stands for molecular weight marker (b–d). Data are presented as mean ± SD of three independent experiments with n = 3. Source data are provided as a Source Data file.