Fig. 1: Engineered display of heterologous proteins or antigen peptides on the OMVs surface. | Nature Communications

Fig. 1: Engineered display of heterologous proteins or antigen peptides on the OMVs surface.

From: Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology

Fig. 1

a Schematic representation of the pET28a-Luc and pET28a-ClyA-Luc construct, and western blot analysis of ClyA-none (CN), Luc, and ClyA-Luc (CL) expression in E. coli Rosetta (DE3), and OMVs derived from the bacteria. b In vitro bioluminescence images to demonstrate luciferase enzyme activity following expression in Rosetta (DE3) and OMVs. c Schematic representation of the pET28a-ClyA-OVA-3HA construct, and western blot analysis of 3× HA-tagged ClyA-OVA (ClyA-OVA-3HA, CO-3HA) expression in E. coli Rosetta (DE3) and OMVs. pET28a-ClyA-OVA-3HA was the expression plasmid, and IPTG was the expression inducer. d TEM image and DLS analysis of ClyA-OVA OMVs (CO OMVs). Scale bar, 100 nm. Source data are provided as a Source Data file.

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