Table 1 Kinetic parameters.
From: Protein kinase A controls the hexosamine pathway by tuning the feedback inhibition of GFAT-1
l-Glu production | d-GlcN6P production | |||||
|---|---|---|---|---|---|---|
Km l-Gln [mM] | kcat [sec−1] | kcat/Km [mM−1 sec−1] | Km Frc6P [mM] | kcat [sec−1] | kcat/Km [mM−1 sec−1] | |
Wild type | 1.1 ± 0.2 | 3.6 ± 0.2 | 3.3 | 0.08 ± 0.01 | 1.7 ± 0.1 | 21.3 |
R203H | 1.6 ± 0.27 | 4.8 ± 0.26 | 3.0 | 0.13 ± 0.02 | 2.0 ± 0.15 | 15.4 |
S205D | 1.3 ± 0.23 | 1.5 ± 0.08 | 1.2 | 0.18 ± 0.04 | 0.8 ± 0.07 | 4.4 |
L405R | 1.0 ± 0.10 | 3.0 ± 0.08 | 3.0 | 0.07 ± 0.02 | 1.9 ± 0.15 | 27.1 |
