Fig. 8: Identification of macula densa cells from captured mouse tubular cells. | Nature Communications

Fig. 8: Identification of macula densa cells from captured mouse tubular cells.

From: Single-cell RNA sequencing reveals the mesangial identity and species diversity of glomerular cell transcriptomes

Fig. 8

a UMAP of captured tubular cells from C57BL/6J mice coloured by cell types and DCT subclusters. CD collecting duct, DCT distal convoluted tubule, PTC proximal tubule cell, MDC macula densa cell, cTAL cortical thick ascending limb. Colour dots indicate diverse subsets. b PAGA graph of cell type topological relations. c Two-dimensional diffusion map trajectory of tubular cells. d The expression (log2-transformed RPKM) of classical MDC markers in tubular cell populations. Colours represent tubular corresponding subsets described in a. e The expression of top 10 genes significantly overexpressed in each identified tubular cell subpopulation. f Double immunofluorescence staining for NT5C1A (green) and SLC29A1 (green) with NOS1 (red) in mouse (left) and human kidney tissues (right). Both proteins NT5C1A and SLC29A1 localize to the same subpopulation of NOS1-positive tubular cells (yellow arrows) next to the glomerulus (G). g Immunostaining for NT5C1A using two different antibodies (HPA HPA050283—left and HPA054158—right) show strong reactivity in a small subpopulation of tubular cells next to the root of the glomerular tuft (arrowheads or arrow). The images are downloaded from www.proteinatlas.org (https://creativecommons.org/licenses/by-sa/3.0/). Scale bars (f, g): 30 µm.

Back to article page