Fig. 2: Phylogeny of BioZ and its paralogs. | Nature Communications

Fig. 2: Phylogeny of BioZ and its paralogs.

From: Biochemical and structural characterization of the BioZ enzyme engaged in bacterial biotin synthesis pathway

Fig. 2

a An unrooted tree of BioZ proteins and its putative homologs (FabH, FabB, and FabF). The phylogenetic tree was generated with the MEGA7 software using the NJ method (bootstrap: 1000 replicates). Two major clades of KAS enzymes are shown: FabF in red and FabH in orange. In contrast, FabB (shown in gray) is phylogenetically positioned as a sub-branch of FabF clade and BioZ was localized to a sub-branch of FabH clade. Therefore, we speculated that FabB originates from FabF (KAS II), and a recent ancestor of BioZ arises from FabH, the KAS III enzyme within the KAS pan-family. b Phylogenetic relationships of BioZ paralogs. Phylogeny was constructed using the MEGA7 software involving the NJ method (bootstrap: 1000 replicates, bootstrap values indicated by circle sizes). BioZ of Agrobacterium and Brucella melitensis are colored in red and blue, respectively.

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