Fig. 5: Structural and functional analyses of the BioZ catalytic triad. | Nature Communications

Fig. 5: Structural and functional analyses of the BioZ catalytic triad.

From: Biochemical and structural characterization of the BioZ enzyme engaged in bacterial biotin synthesis pathway

Fig. 5

a Structural snapshot of the catalytic triad (C163, H298, and H333) of EcFabB. b Structural analysis of the catalytic triad (C164, H304, and H341) of EcFabF. c Structural presentation of the catalytic triad (C112, H244, and N274) of EcFabH. d Structural illustration of the catalytic triad (C115, H255, and N285) of AtBioZ. The figures of structures were generated using the PyMol software. e Functional assays of BioZ and its catalytic triad mutants using the ΔbioH biotin-auxotrophic strain. The tested strains were grown on nonpermissive M9 minimal media lacking biotin. Growth curves were generated from three independent experiments and displayed in an average ± standard deviation (SD).

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