Fig. 2: Synovial fibroblasts from arthritic mice have a reduction in sialylated N-glycans.

N-glycans from synovial fibroblasts expanded from healthy or arthritic synovial fibroblasts were isolated and permethylated prior to MALDI-TOF MS analysis. 43 structures were selected (relative expression >0.02%) and MS peak area was quantified and normalized against total measured intensities. Unsupervised hierarchical clustering with Euclidean distance grouped structures into six main clusters. Structures present in each cluster are shown. Blue and red dots represent SFs isolated from healthy and CIA mice respectively. Data show the results from 4 independent experiments, where healthy and arthritic fibroblasts samples were processed in parallel. Before-after graphs show all structures present in the indicated clusters, where individual dots are the mean of relative expression from 4 experiments and similar structures in healthy and CIA cells are connected with lines. Statistical significance between healthy and CIA was assessed by one-tail paired t-test, *p < 0.05 and **p < 0.01. Actual p-values: a 0.0046, b 0.0371, c 0.024. Relative expression for each glycan structure (shown with their m/z value) is also shown in scatter plots showing mean and SEM, where each dot represents the relative expression of one independent experiment, n = 4, *p < 0.05 and **p < 0.01 using unpaired one-tail t-test. Actual p-values: d 0.0153, e 0.0385, f 0.0372, g 0.0478, h 0.0005, i 0.0420.