Fig. 6: Reduced α2-6-sialylation is restricted to CD90+ synovial fibroblasts.
a Synovial fibroblasts from healthy mice were isolated from mouse joints and identified by flow cytometry as in Fig. 1 (Zombie Violet−, CD45−, CD31−, Podoplanin+), separated into CD90+ and CD90− populations and stained with the biotinylated lectins SNA, MAAII, and PNA, and streptavidin-PE-Cy7. Graphs show the mean fluorescence intensity for each lectin. Data are presented as mean ± SEM, dots represent cells from one individual mouse (n = 3). One-tail unpaired t-test was used for statistics, **p < 0.01, #p < 0.05. Actual p-values: a 0.0264, b 0.023. b Synovial fibroblasts from healthy and arthritic mice (n = 4) were isolated from mouse joints as in a and RNA was purified using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions. Expression of Il6, Mmp3, and St6gal1 mRNA was quantified by RT-qPCR. Data are presented as mean ± SEM. One-tail unpaired t-test was used for statistics, **p < 0.01, #p < 0.05 compared to the same population in control healthy mice. Actual p-values: c 0.0118, d 0.0428, e 0.0103, f 0.0225, g 0.0183.
