Fig. 8: Levels of sialylation correlate with disease stages and pathotypes of human RA. | Nature Communications

Fig. 8: Levels of sialylation correlate with disease stages and pathotypes of human RA.

From: Loss of α2-6 sialylation promotes the transformation of synovial fibroblasts into a pro-inflammatory phenotype in arthritis

Fig. 8

a Synovial fibroblasts were expanded from human biopsies from n = 2 naive RA patients (not exposed to any disease-modifying anti-rheumatic drug) and n = 3 RA patients in sustained clinical and imaging remission achieved with c-DMARDs+TNF-inhibitor combination therapy. N-glycans were isolated and subjected to MALDI-TOF MS analysis. Relative intensity was used to calculate the ratio of expression of the indicated paired glycans. Glycan structures are designated by their m/z value. b Synovium from OA, and naive and remission RA patients were stained with biotinylated SNA and Alexa-647-conjugated streptavidin (magenta). DAPI (cyan) was used to stain cell nuclei. Images were acquired with a confocal microscope LSM 880. Results are from one single experiment, OA n = 1, active RA = 2, remission RA n = 2. Each image represents one individual patient. Scale bars: 200 μm.

Back to article page