Fig. 3: Active site signatures of AA7 oligosaccharide oxidases versus dehydrogenases. | Nature Communications

Fig. 3: Active site signatures of AA7 oligosaccharide oxidases versus dehydrogenases.

From: Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs

Fig. 3

The active sites of the clade IIa discovered dehydrogenase FgCelDH7C (PDB: 6YJI) and the canonical clade Va cellooligosaccharide oxidase SsGOOX (in complex with 5-amino-5-deoxy-cellobiono-1,5-lactam ABL, PDB: 2AXR) are shown. a The FAD-tethering histidine in addition to an aromatic cluster comprising the tyrosine base catalyst, a phenylalanine/tyrosine and the substrate-stacking aromatic residue are conserved features in fungal oligosaccharide dehydrogenases and oxidases. b Sequence logos of patches spanning the structurally similar active site residues shown in (a). c Active site differences between clades IIa and Va. d Sequence logos of patches spanning the structurally divergent active site residues shown in c from clades IIa and Va. The amino acid numbering of the deposited protein sequences is used in the figure.

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