Table 1 Apparent kinetic parameters of AA7-catalysed oxidation of saccharides.

GalNAc

k_cat (s⁻¹)

3.7 ± 0.1

ND

ND

K_M (mM)

1.69 ± 0.12

ND

ND

k_cat/K_M (s⁻¹ M⁻¹)

2.16 × 10³

GlcNAc

k_cat (s⁻¹)

3.8 ± 0.1

3.0 ± 0.2

6.8 ± 0.3

3.3 ± 0.2^a

K_M (mM)

0.80 ± 0.05

0.69 ± 0.20

15.7 ± 1.13

0.50 ± 0.10^*

k_cat/K_M (s⁻¹ M⁻¹)

4.75 × 10³

4.27 × 10³

4.30 × 10²

5.94 × 10^3a

k_cat (s⁻¹)

4.4 ± 0.1

3.8 ± 0.04

2.3 ± 0.03

18.3 ± 0.4^a

28.7 ± 0.4

Chitobiose

K_M (mM)

0.064 ± 0.01

0.3 ± 0.02

0.27 ± 0.01

0.33 ± 0.03^a

1.32 ± 0.07

k_cat/K_M (s⁻¹ M⁻¹)

6.83 × 10⁴

1.27 × 10⁴

8.58 × 10³

5.56 × 10^4a

2.17 × 10^4a

k_cat (s⁻¹)

3.71 ± 0.09

3.55 ± 0.04

6.14 ± 0.29

2.0 ± 0.1^a

Chitotriose

K_M (mM)

0.09 ± 0.01

0.28 ± 0.01

0.25 ± 0.03

0.75 ± 0.1^a

k_cat/K_M (s⁻¹ M⁻¹)

4.22 × 10⁴

1.25 × 10⁴

2.44 × 10⁴

2.67 × 10^3a

k_cat (s⁻¹)

30.8 ± 0.81

36.1 ± 0.74

39.27 ± 0.76

45.28 ± 2.03

K_M (mM)

5.35 ± 0.39

3.94 ± 0.22

6.06 ± 0.27

11.25 ± 0.94

k_cat/K_M (s⁻¹ M⁻¹)

5757

9162

6480

4025