Fig. 4: Temporal changes in inbreeding, relative mutational load and number of loss-of-function (LoF) variants in two populations of Sumatran rhinoceros (Dicerorhinus sumatrensis).
a Inbreeding estimated as the average proportion of the genome in runs of homozygosity (FROH). Open bars show the total proportion of the genome in ROH ≥100 kb and solid bars show proportions in ROH of length ≥ 2 Mb. Bars extending from the mean values represent the standard deviation (two-sided t-test, FROH ≥ 100 kb: p = 0.034, FROH ≥ 2 Mb: p = 0.007). b Mutational load estimated with GERP scores. Individual relative mutational load was measured as the sum of all derived alleles multiplied by their conservation-score over the total number of derived alleles (see ‘Methods’). Only derived alleles above conservation-score of 1 (i.e., non-neutral) were included (two-sided t-test, p = 0.047). c Number of LoF variants using an annotation of 33,026 genes for white rhinoceros (see ‘Methods’) (two-sided t-test, p = 0.35). Middle thick lines within boxplots and bounds of boxes represent mean and standard deviation, respectively. Vertical lines represent minima and maxima. n = 6, **p < 0.01, *p < 0.05, ns = non-significant, p-values were not adjusted for multiple comparisons.
