Fig. 3: MOCCI and miR-147b reduce NDUFA4 expression during inflammation. | Nature Communications

Fig. 3: MOCCI and miR-147b reduce NDUFA4 expression during inflammation.

From: Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity

Fig. 3

a Intracellular flow cytometry staining for MOCCI in untreated (gray line) and IL-1β-treated (red line) human aortic endothelial cells (HAECs). Percentage of MOCCI-positive HAECs is 25.7 ± 5.2% (mean ± se), n = 4 biological replicates per condition. b Co-immunofluorescent staining for MOCCI and TOMM20, a mitochondrial protein in HAECs. Scale bar = 100 µm, n = 3 biological replicates. c C15ORF48 mRNA and miR-147b expression in untreated and IL-1β-treated HAECs. Data are presented as mean ±  SEM, n = 3 biological replicates per condition. d Intracellular flow cytometry staining for NDUFA4 and MOCCI in untreated or IL-1β-treated HAECs. e Intracellular flow cytometry staining for NDUFA4 and MOCCI in untreated or IL-1β-treated A549 cells. f Intracellular flow cytometry staining for NDUFA4 and MOCCI in HAECs overexpressing MOCCI, C15ORF48 WT-mRNA (both MOCCI and miR-147b), and C15ORF48 ATGmut-mRNA (only miR-147b), n = 3 biological replicates per condition. g Levels of C15ORF48 mRNA (primers binding to the 5′UTR of C15ORF48) or miR-147b by qPCR in HAECs with the indicated overexpression. Data were presented as mean ±   SEM, n = 3 biological replicates per condition. h Western blot of NDUFA4 in HAECs with the indicated overexpression. n = 3 biological replicates. Source data are provided as a Source Data file.

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