Fig. 4: NDUFA4 is exchanged for MOCCI in Complex IV during inflammation. | Nature Communications

Fig. 4: NDUFA4 is exchanged for MOCCI in Complex IV during inflammation.

From: Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity

Fig. 4

a Volcano plot of proteins detected by quantitative TMT-mass spec in AAV-GFP or AAV-MOCCI mouse heart mitochondria. p = two-tailed Student’s t test, n = 3 mice per AAV. b BN-PAGE (top) and SDS-PAGE (bottom) of AAV-MOCCI/GFP mouse heart mitochondria probed for the indicated respiratory chain proteins. Each lane represents mitochondria from one mouse. Quantification of NDUFA4 shown in Supplementary Fig. 4a. c Two-dimensional BN-SDS-PAGE to show downregulation of NDUFA4 in CIV complexes. n = 3 biological replicates. Source data are provided as a Source Data file. d Alignment of the predicted secondary structure of MOCCI (iTASSER model) and known structure of NDUFA4 in Maestro (Schrodinger). e The position of NDUFA4 (ribbon) in CIV cryo-EM structure (PDB 5Z62). All other subunits are depicted in tube representation. MTCO-1 (purple) and the redox centers (HEME-A and Cu2+) are highlighted to illustrate the relative position of NDUFA4. f Co-IP of NDUFA4 and MOCCI. Digitonin (1%)-solubilized isolated mouse heart mitochondria were immunoprecipitated with anti-FLAG and probed for the proteins as indicated. AAV-GFP served as negative control. Source data are provided as a Source Data file.

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