Fig. 2: Histamine binding and the ligand-binding pocket.
From: Cryo-EM structure of the human histamine H1 receptor/Gq complex
a The ligand-binding pocket of histamine. The histamine is shown in red in the middle of the pocket, density map of histamine (blue mesh) is set at contour level of 3.0. Surrounding residues within 4.1 Å of histamine are shown in sticks and colored in cyan. Hydrogen bonds are marked as yellow dash line. b A NFAT-RE reporter assay of mutations of key residues in the ligand-binding pocket. Histamine, 10 µM; data are presented as mean values ± SD; n = 3 independent samples. RLU, relative luciferase unit. WT, wild-type. c The electrostatic potential surface of H1R and the histamine binding pocket. The chemical structure of histamine is shown on the up-right panel.
