Fig. 1: Localization of BcCrh1 inside plant cells is required for induction of cell death. | Nature Communications

Fig. 1: Localization of BcCrh1 inside plant cells is required for induction of cell death.

From: The Botrytis cinerea Crh1 transglycosylase is a cytoplasmic effector triggering plant cell death and defense response

Fig. 1

a Plants were infiltrated with Agrobacterium strains that were transformed with the bccrh1 gene with or without secretion signal (SP). Images of necrotic lesions (a) were taken five days after Agroinfiltration. 35S:GFP: free GFP (control); 35S:BcCrh1-SP and 35S:MBcCrh1-SP: native and enzyme inactive BcCrh1 respectively, without secretion signal; 35S:SPPR3-BcCrh121–391 and 35S:SPPR3-MBcCrh121–391: fusion of the native and enzyme inactive BcCrh1 respectively, with PR3 plant signal peptide; b–g Subcellular localization of GFP-fusion proteins. Leaves were harvested two days after Agroinfiltration, submerged for 20 min in 0.8 M mannitol to induce plasmolysis, and then samples were scanned by a confocal microscope. White asterisks mark apoplastic space between the cell wall (black arrow) and plasma membrane (red arrow) in plasmolysed plant cells. Left panel shows images of cells following Agroinfiltration with free GFP (control), right panel shows images following Agroinfiltration with BcCrh1-GFP fusion protein. b GFP without SP; c GFP fused to the BcCrh1 SP; d GFP fused to PR3 SP; e BcCrh1-GFP with native SP; f BcCrh1-GFP fused to PR3 SP; g MBcCrh1-GFP fused to PR3 SP. Bars = 20 μm. All the above experiments were repeated at least three times with similar results.

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