Fig. 2: A stretch of 35 amino acids of BcCrh1 is necessary and sufficient for the induction of plant cell death. | Nature Communications

Fig. 2: A stretch of 35 amino acids of BcCrh1 is necessary and sufficient for the induction of plant cell death.

From: The Botrytis cinerea Crh1 transglycosylase is a cytoplasmic effector triggering plant cell death and defense response

Fig. 2

a Schematic presentation of different BcCrh1 fragments that were used in Agroinfiltration assays. Green—SP, Red—the minimal region that was found to be sufficient for induction of cell death, Blue—other regions of the tested fragment. b–c representative images of leaves following infiltrations with Agrobacterium strains that contain constructs encoding the different BcCrh1 fragments. b Pictures were taken five days after Agroinfiltration; c Leaves were harvested two days after Agroinfiltration, submerged for 20 min in 0.8 M mannitol to induce plasmolysis, and then samples were scanned by a confocal microscope. Typical apoplastic space between the cell wall and plasma membrane in plasmolysed plant cells is marked with red asterisks. Bars = 20 μm. All the above experiments were repeated at least three times with similar results.

Back to article page