Fig. 5: PRNPs selectively accumulate in inflamed joints of rats with advanced arthritis. | Nature Communications

Fig. 5: PRNPs selectively accumulate in inflamed joints of rats with advanced arthritis.

From: Targeted apoptosis of macrophages and osteoclasts in arthritic joints is effective against advanced inflammatory arthritis

Fig. 5

a Ex vivo DiD fluorescence images showing the biodistribution of NPs, RNPs, and PRNPs in AIA rats with advanced arthritis (A, heart; B, liver; C, spleen; D, lung; E, kidney; F, Blood; G, arthritic joint) at 24 h post injection. b In vivo DiD fluorescence images showing the arthritic joint distribution of free DiD, and DiD-loaded NPs, RNPs, and PRNPs in AIA rats with a unilateral inflamed joint at 24 h post injection. c Ex vivo DiD fluorescence images in the inflamed joints and un-inflamed joints from AIA rats with a unilateral inflamed joint at 24 h post injection with free DiD, and DiD-labeled NPs, RNPs, or PRNPs. d The statistical graphs of the fluorescence intensity of inflamed joints based on the semi-quantitative analysis of the ex vivo fluorescence images after i.v. administration of free DiD or DiD-labeled nanoparticles. Data represent mean ± SD (n = 6 inflamed joints from 3 independent animals). Statistical significance was determined by two-sided Student’s t test. e The statistical graphs of the fluorescence intensity of major organs from AIA rats with advanced arthritis after i.v. administration of free DiD or DiD-labeled nanoparticles. Data represent mean ± SD (n = 3 independent animals). Statistical significance was determined by a two-sided Student’s t test. f The statistical graphs of the fluorescence intensity of inflamed joints and un-inflamed joints from AIA rats with a unilateral inflamed joint after i.v. administration of free DiD or DiD-labeled nanoparticles. Data represent mean ± SD (n = 3 independent animals). Statistical significance was determined by a two-sided Student’s t test. DiD 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indodicarbocyanine, 4-chlorobenzenesulfonate salt, DiD-NPs DiD labeled poly (d, l-lactide-co-glycolide) (PLGA) nanoparticles, DiD-RNPs DiD-labeled RGD peptide-modified PLGA nanoparticles, DiD-PRNPs DiD-labeled matrix metalloproteinase 9 (MMP9)-cleavable polyethylene glycol (PEG)- and RGD peptide-modified PLGA nanoparticles.

Back to article page