Fig. 2: Ecotopic expression of MoCel12A increased the blast disease resistance in rice.
a Phenotype of the architecture (left) and the leaf lesions (right) of MoCel12A-expressing plants. Wild-type (WT) and MoCel12A ecotopic-expression plants (MoCel12A-OE) were grown in paddy soil and photographed at the heading stage. WT is empty vector control. OE-3 and OE-5 are two independent transgenic lines. b Phenotype of the architecture (Left) and the leaf lesions (Right) of MoCel12A2ED expressing plants. WT and MoCel12A2ED ecotopic-expression plants were grown as in (a). OE-1 and OE-3 are two independent transgenic lines. c Trypan-blue staining of rice plants ecotopically expressing MoCel12A and MoCel12A2ED. d Rboh and PR gene expression in WT, MoCel12A, and MoCel12A2ED transgenic plants. The leaves of 4-week-old plants were sampled, and RT-qPCR was used to evaluate the gene expression. OsActin served as the internal reference gene. Values are means ± SD (n = 3 biological replicates). e MoCel12A-OE expression plants exhibit enhanced disease resistance to M. oryzae. Left: disease symptoms of WT and MoCel12A-OE leaves infected with Guy11. Conidial suspensions (1 × 105 conidia per ml in 0.02% Tween-20) were sprayed onto the leaf surfaces of 2-week-old rice seedlings. The images were taken at 5 dpi. Bar = 1 cm. Right: the relative fungal biomass determined by qPCR. Values are means ± SD (n = 3 biological replicates). ** indicates significant differences from WT at P < 0.01 (two-sided Student’s t test). f Expression of inactive MoCel12A in rice did not affect the blast disease resistance. Left: disease symptoms of WT and MoCel12A2ED-OE leaves infected with Guy11. Right: relative fungal biomass. Others are as in (e).
