Fig. 7: Structural transitions in DgcR upon activation.

Rec pseudo-phosphorylation induces steps 1 to 3, which are followed by GGDEF hinge motions of steps 4 and 5 to attain the catalytically competent state. See also Supplementary Movies 1 and 2. The structures are represented as in Fig. 1b, c, but with the residues of the conditional coiled-coil contacts shown as CPK models (residues in d and e position are shown is pink and green). The beryllofluoride moieties of the dimer are highlighted by magenta circles. a DgcR’, symmetrized version with both GGDEF domains in B-chain orientation (cf. with Fig. 1b). b As in a, but with beryllofluoride-induced tertiary change applied to Rec rigid_body 1 (see Fig. 3). c As in b, but with quaternary change applied to Rec domains. Note the clash between the C-terminal ends of the coiled-coil (red circle). d As in c, but with Rec dimer as found in symmetrized version of DgcR’*. e Symmetrized version of DgcR’* (cf. with Fig. 1c). f Model of catalytically competent DgcR as in Fig. 6b.