Fig. 3: Biologically relevant information is contained in uTAR features. | Nature Communications

Fig. 3: Biologically relevant information is contained in uTAR features.

From: Uncovering transcriptional dark matter via gene annotation independent single-cell RNA sequencing analysis

Fig. 3

Differential uTAR feature analysis for mouse spleen data (a), chicken heart day 4 data (b), gray mouse lemur EPCAM+ lung data (c), naked mole rat spleen data (d), and sea urchin embryo data (e). Dot plot (left) of differentially expressed uTAR features that are labeled based on sequence homology and cell clusters are numbered along the x-axis. Dot size corresponds to the percentage of cells that express the uTAR feature while darker blue color corresponds to higher level of log-e-normalized expression. UMAP (second left) colored and dimensionally reduced using gene expression features where cell clusters are labeled above the UMAP. Total coverage plot (top) of 5 uTARs along the length of the uTAR feature on the x-axis. The corresponding feature plot on UMAP projection is shown below the coverage plots where darker brown color correlates with higher log-e-normalized expression in each cell.

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