Fig. 2: Genome-wide profiling of cell-type- and AGO-specific miRNAs.
a Volcano plot showing all reference miRNAs enriched (log2 fold change > 2, P < 0.05 relative to wild type) in immunoprecipitations of HAALG-1 or HAALG-2 in one or more cell-type (red). P value was calculated by fitting a two-tailed negative binomial model to processed read counts, according to edgeR pipeline for pairwise comparisons between multiple groups. b Venn diagram representation of all AGO-loaded (either ALG-1 or ALG-2) cell-type-specific miRNAs in intestine (blue), neurons (green) or body wall muscles (BWM, pink). c Venn-diagram depicting cell-type-specific and AGO-type-specific loading of miRNAs (ALG-1—top and ALG-2—bottom). d Heatmap showing unsupervised clustering of ALG-1 or ALG-2 cell-type-specific loading of miRNAs. e Sensitivity of cell-type-specific immunoprecipitation demonstrated by detection of miR-791 expressed from three neurons (right—fluorescence image of miR-791p::GFP). Scale bars, 50 µm. f Enhanced sensitivity of cell-type-specific immunoprecipitation demonstrated by detection of lsy-6 expressed from a single neuron (right—fluorescence image of lsy-6p::GFP). e, f Graph shows reads per million in indicated cell types of two biological replicate experiments, two bars. Scale bars, 50 µm.
