Fig. 7: Specific effects of alg-2 on the neuronal “translatome”. | Nature Communications

Fig. 7: Specific effects of alg-2 on the neuronal “translatome”.

From: Cell-type-specific profiling of loaded miRNAs from Caenorhabditis elegans reveals spatial and temporal flexibility in Argonaute loading

Fig. 7

a Schematic representation of cell-type-specific polysome immunoprecipitation setup. Single copy FLAG-tagged RPL-18 protein driven by the neuron-specific rgef-1 promoter allows purification of neuron-specific translating mRNAs from either wild-type or alg-2 mutant backgrounds. Scale bars, 50 µm. b Scatterplot showing expression levels (log2 CPM, counts per million) of mRNAs immunoprecipitated with polysomes from neurons in alg-2 mutant compared to wild-type worms. Red points represent significantly differentially upregulated or downregulated genes with a FDR value of <0.01. Fold change and significance was calculated by fitting a two-sided negative binomial model to processed read counts, according to edgeR pipeline for pairwise comparisons between multiple groups. c Broad gene ontology categorization of mRNAs upregulated (left) or downregulated (right) in neurons of alg-2(ok304).

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