Fig. 4: KDM4B activity inhibits the ALT pathway.
From: Mutations inhibiting KDM4B drive ALT activation in ATRX-mutated glioblastomas
Immunostaining of KDM4B (green), PML (red) and TEL-FISH (cyan) in control or KDM4B transfected B143 and U2OS cells. Arrows show KDM4B at telomeres (a). Percentages of co-localised TEL-FISH and PML foci in H3.3G34RAPT-tKO#1 cells are shown in b (n = 3 independent experiments; >500 foci counted). Immunostaining of γH2AX (red; diluted at 1/500) and TEL-FISH (green) analyses in B143 and U2OS cells with and without overexpression of KDM4B (+KDM4B). Arrows show γ-H2AX at telomeres (c). Percentages of co-localised TEL-FISH and γH2AX foci are shown in d. For each cell line, >1500 telomeric or TEL-FISH telomeric foci were counted (n = 4 independent experiments). Percentage of co-localised TEL-FISH/γH2AX foci are determined as percentages of telomeric foci that co-stained with γH2AX over the total number of telomeric foci counted. b, d Data are presented as mean values ± SD. **indicates p < 0.005, Student’s t-test with two-tailed distribution. a, c Scale bars: 5 μm; representative images from at least n = 3 independent experiments. Source data are provided with this paper.
