Fig. 6: Activation of the Schengen-pathway represses water transport and maintains plant growth, survival, and fitness under fluctuating environment.
From: Two chemically distinct root lignin barriers control solute and water balance
a Boxplot showing the hydrostatic root hydraulic conductivity (Lpr−h) in WT, sgn3-3, myb36-2, sgn3-3 myb36-2 grown hydroponically for 19–21 days under environmental controlled conditions. Hydraulic conductivity was measured using pressure chambers (Lpr−h). Different letters indicate significant differences between genotypes determined by an ANOVA and Tukey’s test as post hoc analyses (p < 0.01, n = 20 for WT, n = 12 for sgn3-3, n = 15 for myb36-2 and n = 11 for sgn3-3 myb36-2). Centre lines show the medians; box limits indicate the 25th and 75th percentiles. The experiment was repeated two times independently with similar results. b Heatmap of aquaporins expression across the different genotypes and treatments used in the RNA-seq experiment. c Representative pictures of WT, sgn3-3, myb36-2, sgn3-3 myb36-2, WT—pELTP::CDEF and sgn3-3 myb36-2—pELTP::CDEF plants germinated in soil with a high humidity (80%) for 7 days and then transferred in an environment with a lower (60% RH) or with constant humidity (80% RH). Pictures were taken 0, 2, 5 and 8 days after the transfer. Scale bar = 1 cm. The experiment was repeated two times independently with similar results. d Boxplots showing the proportion of dead plants after transfer in an environment with constant humidity (80% RH, blue) or with a lower (60% RH, red). The plants displaying no growth after 9 days and showing necrosis in all the leave surface were considered as dead plants. Each point represents the proportion of dead plants in a cultivated pot compared to the total number of plants for one genotype in the same pot. Pots were containing at least eight plants of each genotypes, n = 10 pots. Different letters represent significant differences between genotypes using a two-sided Mann–Whitney test (p < 0.01). centre lines show the medians; box limits indicate the 25th and 75th percentiles. e Boxplots showing the number of siliques produced per plants. Plants were cultivated in a high humidity environment for 10 days after germination and then transferred to a greenhouse. Each point represents the total number of seeds containing siliques per plant (n = 12 for WT, n = 14 for sgn3-3, n = 12 for myb36-2, n = 11 for sgn3-3 myb36-2, n = 12 for WT—pELTP::CDEF, n = 15 for sgn3-3 myb36-2—pELTP::CDEF). Different letters represent significant differences between genotypes using a two-sided Mann–Whitney test (p < 0.01). Centre lines show the medians; box limits indicate the 25th and 75th percentiles.
