Fig. 4: Rosa26 (R26) BirA*G3–ER and R26 BirA*G3–ER;Cre 3A mouse embryonic stem cell (mESCs) characterization.
From: Proteomics of protein trafficking by in vivo tissue-specific labeling
a Schematic showing Cre-induced recombination of the R26 BirA*G3-ER allele to generate the BirA*G3-ER;Cre expressing cell line. GFP is in blue, BirA*G3-ER-myc is in black, mKate2 is in red, homologous regions to the R26 locus are in blue, and other elements are colored in gray. b–i Native immunofluorescence in BirA*G3-ER parental (GFP+ (green)/mKate2−) or Cre-recombined (GFP−/mKate2+(red)) ESC colonies. DAPI (nuclei) is in blue. Representative images of four repeats. Scale bar: 100 µm. j, k Western blot analysis of mESC whole cell lysate with or without Cre recombination. j Total protein stain (red). k Western blot probed with streptavidin (cyan) or an anti-myc (red) antibody to detect myc-tagged BirA*G3. For each image, all lanes are from the same blot (see Source Data for uncropped blots). Representative results of three western blots. See also: Supplementary Fig. 11. Source data are provided as a Source Data file.
