Fig. 2: Talin regulates linear force-FAK signaling coupling at FAs. | Nature Communications

Fig. 2: Talin regulates linear force-FAK signaling coupling at FAs.

From: Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair

Fig. 2

a–d Heatmaps of tFAK and pY397-FAK intensities at FAs for cells treated with (a, b) control or (c, d) talin-1 shRNA. Scale bar 5 µm. e Total traction force (mean ± SD) for control shRNA-treated and talin-1 shRNA-treated cells. Two-sided Mann–Whitney test α: P = 0.0005, n = 34 cells for each condition. f tFAK intensity (mean ± SD) at individual FAs. Two-sided Mann–Whitney test α: P < 0.0001, n = 40 and 42 FAs for control and talin-1 shRNA, respectively. g pY397-FAK intensity (mean ± SD) at individual FAs. Two-sided Mann–Whitney test α: P < 0.0001, n = 51 and 42 FAs for control and talin-1 shRNA, respectively. h Traction force vs. tFAK intensity at individual FAs. Linear regression: control shRNA P < 0.0001, n = 40 FAs, tFAK intensity = 96.1 × force + 1712; talin-1 shRNA P = 0.6061, n = 42 FAs. i Force vs. pY397-FAK intensity at individual FAs. Linear regression: control shRNA P < 0.0001, n = 51 FAs, pY397-FAK intensity = 268.3 × force + 3056; talin-1 shRNA P = 0.2421, n = 42 FAs. j Schematic of FAK constructs. k Force vs. tFAK intensity at individual FAs for WT FAK. Linear regression P < 0.0001, n = 70 FAs, tFAK intensity = 110.2 × force + 1779. l Force vs. pY397-FAK intensity at individual FAs for WT FAK. Linear regression P < 0.0001, n = 68 FAs, pY397-FAK intensity = 150.0 × force + 3776. m Force vs. tFAK intensity at individual FAs for E1015A FAK. Linear regression P = 0.0607, n = 65 FAs. n Force vs. pY397-FAK intensity at individual FAs for E1015A FAK. Linear regression P = 0.0619, n = 78 FAs. o Force vs. tFAK intensity at individual FAs for K454R FAK. Linear regression P < 0.0001, n = 70 FAs, tFAK intensity = 142.1 × force + 1607. p Force vs. pY397-FAK intensity at individual FAs for K454R FAK. Linear regression P = 0.0610, n = 48 FAs. q Force vs. tFAK intensity at individual FAs for Y397F FAK. Linear regression P < 0.0001, n = 65 FAs, tFAK intensity = 135.1 × force + 1525. r Force vs. pY397-FAK intensity at individual FAs for Y397F FAK. Linear regression P = 0.1869, n = 60 FAs.

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