Fig. 6: C3-domain condensation assays. | Nature Communications

Fig. 6: C3-domain condensation assays.

From: Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity

Fig. 6

a Scheme of the condensation reaction using PCP2C3 SpyCatcher and Spytag-PCP3 constructs. b Level of tetrapeptide formation demonstrated by the WT C3-domain with or without SpyCatcher and SpyTag; the reaction was performed using a DHB-d-Arg-Gly donor substrate and a Gly acceptor substrate. c Level of tetrapeptide formation by WT and different C3-domain mutants using BA-d-Arg-Gly as a donor substrate and different aminoacyl acceptor substrates. All reactions performed in triplicate, unless specifically stated (* single reaction; # duplicate); see Supplementary Figs. 30–35 for traces; for data see [http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD024004].

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