Fig. 1: Arginine globally modulates metabolic gene expression via histone acetylation.

a Heat map of Affymetrix microarray analysis shows arginine (Arg+) globally induced metabolic pathways, including oxidative phosphorylation pathway, glucose, fatty acid, and DNA metabolic pathways in CWR22Rv1 prostate cancer cells. The color key indicates the fold change to control. b After starvation overnight, cells were treated with arginine and then harvested at different time points for total acetyl-histone H3 ELISA assay. This data shows that arginine regulated the total level of histone H3 acetylation at different time points. c After starvation overnight, cells were treated with arginine and then harvested at different time points for total acetyl-CoA assay. ELISA data show that arginine regulated the total level of acetyl-CoA at different time points. d Heat map of acetylated histone H3 ChIP-seq peaks showing that the distribution of peaks are close to transcription star sites (TSS). e Overlap of arginine stimulation Arg (+) and arginine deprivation Arg (−) ChIP-seq peaks. f Acetylated Histone H3 ChIP-sequencing peaks classified by human genomic annotation (hg38), showing that the peaks of histone acetylation are mainly enriched in the promoter region of genes. Data are presented as mean values ± SEM of independent experiments (n = 3 in b, c). **p < 0.01, ***p < 0.001, ****p < 0.0001, using unpaired two-tailed Student’s t test. Source data are provided as a Source data file.