Fig. 2: Arginine epigenetically modulates mitochondrial OXPHOS genes.
From: Arginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells
a Ingenuity pathway analysis on metabolic pathways shows arginine stimulation epigenetically modulates key metabolic pathways, including plasma membrane biosynthesis, DNA synthesis, and OXPHOS pathways. b Ingenuity pathway analysis on cell signaling pathways shows arginine stimulation mainly induced mTOR pathway (highlighted in blue). c ChIP-seq distribution for acetylated histone H3 at representative OXPHOS genes loci. d The summary list of OXPHOS genes enriched in the arginine-stimulation group based on ChIP-seq data. The upregulated genes in microarray data are marked with an asterisk (*). e ChIP-qPCR of histone H3 acetylation confirmed arginine stimulation induced histone acetylation on the promoter region of OXPHOS genes. The value indicated the fold enrichment after arginine stimulation. f After starvation, cells were treated with arginine for 24 h and then harvested. Both total lysate and mitochondrial fraction (Mito) were used for immunoblotting. These data show that arginine induced OXPHOS protein expression. g After starvation, cells were treated with arginine for 24 h and then harvested for individual complex activity assay. These data show arginine stimulation increased mitochondrial complex activities. Data are presented as mean values ± SEM of independent experiments (n = 3 in e, g). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, using unpaired two-tailed Student’s t test. Source data are provided as a Source data file.
