Fig. 3: Transamination of model peptides with different N-termini.
From: Modification of N-terminal α-amine of proteins via biomimetic ortho-quinone-mediated oxidation
a Landscape for the transamination of 20 canonical amino acids attached on the N-terminal residue of the model peptides (Error bars represent standard deviation, n = 3 independent replicates). Reaction conditions: (1) Transamination: Ox1 (10 mM, sat.), 200 mM Na2HPO4, pH 6.5, 3 h, 25 °C; (2) Oxime formation: EtONH2·HCl (100 mM), pH 4.0, 3 h, 25 °C (oxime formation time for N(16)*: 5 h; b A summary of major unexpected products of transamination at different N-termini with Ox1. Structures of these products were characterized with 2D NMR.
