Fig. 2: Cell types assigned to each cluster based on marker gene expression.
From: Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli
a The cell types and the gene markers from Fig. 1b, c used to assign the cell type are presented. The colors in the key correspond to the cluster colors from Fig. 1a. ICs were identified by ATP6V1G3 (a subunit of V-ATPase) expression and consisted of clusters 0, 1, 3, 5, 11, and 12. A-ICs were identified by SLC4A1 expression and consisted of 0, 3, 5, and 7. B-ICs were identified by SLC26A4 expression that was limited to cluster 11. PCs were identified by AQP2 expression which was prominent in clusters 7 and 10. While cluster 10 appeared to be traditional PCs, cluster 7 were hybrid PCs–ICs that expressed both AQP2 and SLC4A1. SLC8A1 was highly selective to hybrid PC–ICs that also expressed CALB1, indicating that these cells might originate in the connecting segment. There were three subtypes of A-ICs that were characterized by HSPA1A and EGR1 expression: A-IC subtype A/cluster 0 expressed HSPA1A, A-IC subtype B/cluster 3 expressed both HSPA1A and EGR1, A-IC subtype C/cluster 5 expressed neither HSPA1A nor EGR1. b The expected location of the cell types within the kidney is presented.
