Fig. 4: Murine-collecting duct cell-type marker expression patterns are generally conserved in human collecting duct cells.

IC markers include the D2, C2, B1, and G3 subunits of V-ATPase (ATP6V0D2, ATP6V1C2, ATP6V1B1, and ATP6V1G3 respectively), carbonic anhydrase 12 (CA12), and forkhead box protein I1 (FOXI1). A-IC markers include aquaporin 6 (AQP6), SLC4A1, adhesion G protein-coupled receptor F5 (ADGRF5), and solute carrier family 26 member 7 (SLC26A7). B-IC markers include insulin receptor-related receptor (INSRR), SLC26A4, and solute carrier family 4 member 9 (SLC4A9). PC markers include AQP2, calbindin 1 (CALB1), FXYD domain containing ion transport regulator 4 (FXYD4), and SCCN1G. Homeobox D10 (HOXD10) is a nephron lineage marker. A dot plot of murine-collecting duct cell-type marker gene expression in human kidney cells is presented. Collecting duct cell types are shaded. Murine markers for ICs and A-ICs are expressed in all human ICs of the same subtypes. The murine B-IC marker SLC4A9 is expressed in human A-IC subtypes A and B along with human B-ICs. Murine PC marker genes are primarily limited to PCs and hybrid PC–ICs in humans, except for FXYD4, which is also expressed in some A-IC subtypes. The majority of human collecting duct cells in this analysis were HOXD10 negative, indicating ureteric origin. PCs and PCs–ICs were the cells that had the highest percentage of HOXD10 positivity indicating derivation from the distal nephron. However, nephron-derived cells still represented a minority of PCs and PCs–ICs. Saline-exposed cell results are presented.