Fig. 3: Opposing actin-isoform dynamics between the cell pole and equator are required for cytokinetic furrow ingression and successful cell division.
From: Inhibition of polar actin assembly by astral microtubules is required for cytokinesis
a Lifeact-GFP signal diminishes at the poles of dividing cells. Boxed region is magnified in the lower panels and the Lifeact-GFP signal on the cortex within the boxed region was quantified. Bars denote the average, whiskers denote ±SD, *p = 0.0003 using nonparametric two-tailed Mann-Whitney t-tests. n = 10 cells examined in each live-cell imaging group. b Relative depth of furrow ingression over time and the comparative rate of furrow ingression in different conditions. *p = 0.03, **p = 0.008, and ***p < 0.0001 using nonparametric two-tailed Mann-Whitney t-tests. n = 5 cells examined in each live-cell imaging group. Dots = average (left panel), bars = average (right panel); whiskers denote ±SD. DIA1 = DIAPH1, CT = C-terminus. c Frames from phase contrast time-lapse series in cells depleted of DIAPH1 or DIAPH3 in the presence or absence of expressed constitutively active DIAPH1 targeted to the plasma membrane (pm). Arrows point to cell equator. Scale bar is 10 μm.
