Fig. 4: Astral microtubules are required for polar γ-actin clearance during anaphase.
From: Inhibition of polar actin assembly by astral microtubules is required for cytokinesis
a HeLa cells fixed and probed with antibodies recognizing tubulin (green), γ-actin (red), Aurora-A (blue), and DAPI to stained chromosomes in white. Cells treated with Centrinone B lack a centrosome at one spindle pole (absence of Aurora-A) and astral microtubules. At this pole cortical γ-actin is maintained during anaphase. b Ratio of γ-actin and DIAPH1 fluorescence intensity between poles of cells (+astral microtubules:−astral microtubules). Bars denote the average, whiskers denote ±SD. **p < 0.0001, *p = 0.0004 using nonparametric two-tailed Mann-Whitney t-tests for three experimental repeats, n = 50 cells examined over three independent experiments. c Pole to pole line-scans along the plasma membrane of representative γ-actin stained cells. One cell is DMSO treated and has a centrosome at each spindle pole, the other is Centrinone B-treated with a centrosome at only one spindle pole. d Cells poles lacking astral microtubules have fewer plasma membrane blebs. Bars denote the average, whiskers are ±SD for three experimental repeats, n = 50 cells examined over three independent experiments. Scale bars are 10 μm.
