Fig. 2: Promoters with high nucleosome occupancy and TSS MNase sensitivity are highly expressed.
From: Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells
a For the top quartile of expressing genes in MCF-10A cells, total mTSS-seq nucleosomes and MNase sensitivity, and immunoprecipitated H2A.Z nucleosomes and H2A.Z MNase sensitivity were sorted based on their expression. Average profiles are shown below. The yellow peak shows the MSN at the TSS. The blue inverted peak shows the MRN at the +1 position. b Alluvial diagram of how the silent cluster 2 and repressed cluster 5 change following their activation when H2A.Z is depleted from MCF-10A cells. c Alluvial diagram showing the fate of active promoter clusters following their inhibition of transcription when H2A.Z is depleted from MCF-10A cells. Line thickness is proportional to the number of promoters that change. d gene expression box plots for the different MCF-10A and shH2A.Z MNase-sensitive promoter classes. Box plots represent the data in quartiles with the median shown as a notch, with the middle 50% of the data contained in the box, and the minima and maxima shown with the upper and lower whiskers representing Q1 and Q4. RNA-seq was performed in three independent biological replicates.
