Fig. 1: Expression of Sox9 in the mouse and human endothelium.
a Whole-mount aorta staining demonstrating SOX9 nuclear staining among endothelial cells (Sox9 in yellow, CD31 in red). b Whole-mount skin staining demonstrating SOX9 expression in dermal vasculature (white arrow; SOX9 positive endothelial cells), immunofluorescent staining of skin sections collected from the Cdh5-CreER RosaYFP mice displays nuclear SOX9 expression in a YFP + endothelial cell. c FACS sorting gating strategy, endovascular progenitor (EVPs) and mature differentiated endothelial (D) cells were FACS sorted and cytospun for staining. FACS sorted EVP showing positive co-staining for ERG, and SOX9 as well as RBPJ with SOX9. D cells are only positive for ERG and RBPJ but not SOX9. Quantification demonstrates a significantly larger percentage of EVPs are positive for RBPJ and SOX9 compared to D cells (***p < 0.005; n = 3; cell sorted from three biologically independent animals; mean ± SD; p value was calculated by two-way ANOVA with multiple comparison of row mean) d Immunofluorescence staining of day 1 (D1) wound sections collected from Cdh5-CreER RosaYFP mice show that YFP + yellow cells within the centre are SOX9 positive.
