Fig. 2: SOX9 expression is essential for endovascular progenitor clonogenicity and quiescence. | Nature Communications

Fig. 2: SOX9 expression is essential for endovascular progenitor clonogenicity and quiescence.

From: Sox9 and Rbpj differentially regulate endothelial to mesenchymal transition and wound scarring in murine endovascular progenitors

Fig. 2

a Schematic diagram demonstrating experimental set up using conditional vascular specific Sox9 knockout mice (Sox9fl/fl/Cdh5-CreER RosaYFP – Sox9eKO) and sample collection 12 weeks post-induction. b Flow cytometry plots showing the aortic endothelium harbour a distinct CD34 positive, lineage (Lin) negative population (red gate) that is entirely YFP positive, as determine by fluorescence minus one (FMO) analysis. Three distinct populations were observed based on CD31 and VEGFR2 expression (from left to right endovascular progenitor EVP; transit amplifying TA; differentiated D) showing the endothelial hierarchy and changes between wild-type controls (Sox9eWT) and Sox9eKO mice after 10 days tamoxifen. c Number of EVP is significantly reduced in Sox9eKO (p < 0.001 vs Sox9eWT; n = 3 biologically independent animals; mean ± SD). d qPCR analysis of genes associated with endothelial quiescence in EVP (***p < 0.001 vs Sox9eWT; n = 3; mean ± SD).

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