Table 1 Experimental and reconstruction details for SAXS-TT scans.
C57BL/6 brain (mouse) | Spinal cord (mouse) | V1 cortex (human) | CC splenium/body (human) | Rag2−/−(sh−/−) brains (mouse) | |
|---|---|---|---|---|---|
Beamline, synchrotron | cSAXS, SLS | LiX, NSLS-II | 4-2, SSRL | cSAXS, SLS | LiX, NSLS-II |
Tube diameter (mm) | 10 | 3 | 20a | 10 | 10 |
Beam energy (keV) | 16.3 | 15.7 | 17 | 16.3 | 16.9 |
Beam size (μm2) | 150 × 150 | 100 × 100 | 250 × 250 | 150 × 150 | 120 × 120 |
Motor step size (μm) | 150 | 100 | 250 | 150 | 120 |
Field of view (pixels) | 69 × 105 | 38 × 61 | 85 × 90 | 70 × 82 | 91 × 41 |
Field of view, size (mm2) | 10.35 × 15.75 | 3.8 × 6.1 | 21 × 22.5a | 10.5 × 12.3 | 10.92 × 4.92 |
No. of projections | 267 | 60 | 151 | 254 | 181 |
Rotation axis tilt angles (°) | 0, 10, 15, 20, 30, 40, 45, 50 | 0 | 0 | 0, 10, 20, 30, 40, 50 | 0 |
Nr of projections/tilt | 67, 34, 32, 32, 30, 26, 24, 22 | 60 | 151 | 66, 56, 50, 44, 38 | 181 |
Exposure time/frame (ms) | 125 | 300 | 120 | 80 | 100 |
Total exposure time (h) | 67.1 | 11.6 | 38.5a | 25.3 | 18.8 |
Total scan time (h) | 87.2 | 17.4 | 61a | 36.8 | 24.8 |
Doseb (Gy) | 2.33 × 104 | 9.96 × 104 | 1.1 × 104 | 1.38 × 104 | 2.48 × 104 |
Reconstruction iterations | 10,000 | 2,000 | 5,000 | 5,000 | 5,000 |
