Fig. 1: MAEA expression is enriched and required in haematopoietic stem cells (HSCs) for haematopoiesis.

a Representative histograms showing MAEA expression on lineage- Sca-1 + c-kit+ progenitor cells (LSKs) and HSCs in control (Ctrl) and Maea^Csf1r-Cre bone marrow (BM). b FACS quantification of MAEA expression on control (n = 5) and Maea^Csf1r-Cre (n = 4) BM HSPCs (LMPP lymphoid-primed multipotent progenitors, CMP common myeloid progenitors, CLP common lymphoid progenitors, GMP granulocyte-macrophage progenitors, MEP megakaryocyte-erythrocyte progenitors), neutrophils (Neu), B cells (B) and monocytes (MN). Data are represented as boxes-and-whiskers with the whiskers span from minima to maxima of each dataset, the boxes extend from the 25th to 75th percentiles and the centre line indicates the mean. HSC p = 0.0202, LSK p = 0.0004, LMPP p = 0.0076, CMP p = 0.0016, CLP p = 0.0173. c The Kaplan–Meyer survival curve of control (n = 8) and Maea^Csf1r-Cre (n = 6) mice. p value was calculated by log-rank test. d White blood cell (WBC) counts in peripheral blood (n = 6 each group) and frequency of B, T and myeloid cells in total WBCs of young adult control and Maea^Csf1r-Cre mice (n = 8 each group). WBC p = 0.0001, B, T and M p < 0.0001. e Quantifications of HSC and LSK numbers in BM of control (n = 6) and Maea^Csf1r-Cre mice (n = 7). HSC p = 0.0165, LSK p = 0.024. f Quantification of myeloid progenitors in BM of control (n = 6) and Maea^Csf1r-Cre (n = 9) mice (MkP: n = 4). CMP p = 0.0146, GMP p = 0.0186, MkP p = 0.0499. g Quantification of lymphoid progenitors in BM of control (n = 6) and Maea^Csf1r-Cre (n = 9) mice^. LMPP p = 0.0027, CLP p = 0.0003. h Experimental scheme and results for evaluating lymphoid differentiation potential of HSC and LMPP at single cell level (HSC: n = 3, LMPP: n = 4 each group). p = 0.025. i Peripheral blood donor chimaerism at indicated time points after competitive BM transplantation (BMT) of equal number (1 × 10⁶) of CD45.1 wild-type (WT) competitor BM cells and CD45.2 donor BM cells from indicated genotypes into lethally irradiated CD45.1 WT recipients (fl/fl: n = 4, fl/+; Cre: n = 5; fl/fl; Cre: n = 6). 1.25 week p = 0.45, 2.25 week p = 0.027, 4 week p = 0.006, 8, 12 16 weeks p < 0.0001. j HSC donor chimaerism in the recipient BM from i at 16wks after transplant (n = 6 each group). p < 0.0001. All data are shown as mean ± sem and *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by unpaired two-sided Student’s t test unless otherwise indicated.