Fig. 3: MAEA regulates cytokine receptor ubiquitination and stability in hematopoietic stem cells (HSCs).
From: MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells
a Representative immunofluorescence images showing MAEA and CD150 expression in HSCs using cells from two independent experiments of four mice. Scale bar = 10 μm. b Representative histograms and FACS evaluation of Flt3 half-life in control and MaeaCsf1r-Cre LSK cells incubated in the presence of 50 μM cycloheximide (n = 4 animals). *p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA multiple comparisons. exact p value from left to right are 0.0325, 0.0016, 0.0003. c Experimental scheme and quantification of HSCs in control (n = 9) and MaeaMx1-Cre (n = 10) mice treated with vehicle and RTK inhibitor (RTKi) PKC412 for 3 weeks after poly I:C induction. p = 0.0212. d Quantification of the frequencies of G0 phase HSCs in control and MaeaMx1-Cre (n = 7 each) mice treated with vehicle and RTKi PKC412 at 3 weeks after poly I:C induction. p = 0.0033. e Quantification of BM macrophages in control (n = 9) and MaeaMx1-Cre (n = 10) mice treated with vehicle and RTKi PKC412 at 3 weeks after poly I:C induction. p value from left to right are 0.0007, 0.0012. All data are shown as mean ± sem. ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by unpaired two-sided t-test with 95% confidence level unless otherwise indicated.
