Fig. 1: Isoform-specific expression of LpR1 is detected in the LNvs. | Nature Communications

Fig. 1: Isoform-specific expression of LpR1 is detected in the LNvs.

From: Brain-specific lipoprotein receptors interact with astrocyte derived apolipoprotein and mediate neuron-glia lipid shuttling

Fig. 1

a A schematic illustration of long and short isoforms of the LpR1 receptor. Top: exon organization of the LpR1 gene. Untranslated regions are in light blue, coding exons are in dark blue. Flexible exons are outlined with orange borders. Bottom: protein domains of LpR1. Long and short-isoforms differ in their N-terminal regions (gray bars) and the numbers of LA modules (green circles). b Exon-mapping of RNA-seq data reveals that only LpR1-short isoforms are expressed in LNvs in both light: dark (LD) and constant light (LL) conditions. Top: schematic representation of the LpR1 gene structure. Bottom: Reads from LNv-specific and CNS RNA-seq libraries mapped to each exon of the LpR1 gene. The lower panel shows a magnified view of the flexible exons and the corresponding LpR1-short isoforms. Untranslated regions are in light blue, coding exons are in dark blue, and flexible exons are outlined with orange borders. Reads number is log scaled. Top Right: isoform-specific FISH probes targeting LpR1-short (red bars) and LpR1-long (black bars) specific sequences. c A diagram illustrating the workflow for qFISH with dissociated brain cells. d Only LpR1-short is expressed in LNvs and is upregulated by excessive visual input in the LL condition. Quantifications of the transcript levels (# dots/cell by qFISH) of LpR1-short and LpR1-long in LNvs from LD and LL conditions are shown. Data are presented as mean values +/− SEM. Statistical significance was assessed by one-way ANOVA with Tukey’s post hoc test. ANOVA: p < 0.0001, F = 143.3, df = 78; short-LD/short-LL: p < 0.0001; long-LD/long-LL: p > 0.9999. n = 20, 22, 20, and 20 for short-LD, short-LL, long-LD, and long-LL, respectively. n represents individual LNvs. ***p < 0.001. e Representative confocal images of qFISH results on single LNvs are shown (observed in at least 20 individual cells). LpR1-short transcripts (red dots) are detected in LNvs (stained by anti-PDF in green). Nuclei are stained by DAPI (blue).

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