Fig. 2: Cell proliferation upon Ambra1 deletion.
a Representative IHC staining of Ki67 on FFPE-tumor sections of BPA+/+ (n = 7) and BPA−/− (n = 7) mice. b Quantification of the Ki67+ cells shown in a. Each data point represents one mouse and corresponds to the average ratio ± SEM of Ki67+ cells on nuclei count (at least three sections analyzed for each mouse) (*p = 0.0262, two-tailed unpaired t-test, BPA−/− vs. BPA+/+). c Representative IHC staining of Ki67 on tumors collected at Breslow ≤2 mm (average = 1.79 ± 0.28 mm; BPA+/+ n = 3, BPA−/− n = 3) and ≥4 mm (average = 4.24 ± 0.69 mm; BPA+/+ n = 7, BPA−/− n = 7). d Quantification of Ki67+ cells shown in c. Each data point represents one mouse and corresponds to the average ratio ± SEM of Ki67+ cells on nuclei count for each mouse (**p = 0.0045 BPA+/+ ≥ 4 mm vs. BPA+/+ ≤ 2 mm; **p = 0.0015 BPA−/− ≥ 4 mm vs. BPA−/− ≤ 2 mm, two-way ANOVA). e Representative WB analysis and quantification (box plot) of Cyclin D1 in BPA+/+ (n = 4) and BPA−/− (n = 4) bulk tumor lysates. ß-tubulin was used as loading control (****p < 0.0001, two-tailed unpaired t-test, BPA−/− vs. BPA+/+). f Representative IHC staining of Cyclin D1 and p-c-Myc-S62 in FFPE-tumor sections of BPA+/+ (n = 6) and BPA−/− (n = 6) mice. g Quantification of Cyclin D1 and p-c-Myc-S62 signals shown in f (*p = 0.0117; ***p = 0.0002, two-tailed unpaired t-test, BPA−/− vs. BPA+/+). Box-and-whisker plots shown in b, e, g represent values from minimum to maximum. Top and bottom whiskers denote upper (Q3) and lower (Q1) quartiles, respectively. Boxes refer to interquartile ranges. Medians are denoted as horizontal line in the middle of the boxes.
