Fig. 2: Detection of SARS-CoV-2 viral RNA and protein antigens in kidney tissues.
From: Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection
a RNA in situ hybridization (ISH) in serial sections of kidney tissues from COVID-19 post-mortem (case #2) showed reactivity for the presence of SARS-CoV-2 viral RNA and the positive control probe to the housekeeping gene peptidylprolyl isomerase B (PPIB). Scale bars = 100 μm. b The kidney tissues from COVID-19 post-mortem (case #2) were incubated with primary anti-SARS-NP (nucleocapsid protein) antibodies (clone ID: 019, rabbit IgG; Sino Biological), anti-SARS-NP antibodies (ab273434, mouse monoclonal 6H3, Abcam), anti-SARS-S (spike) antibodies (ab273433, mouse monoclonal 1A9, Abcam), or only incubated with mouse or rabbit IgG1 control antibodies, and the expression of indicated viral antigens were further analyzed by immunohistochemistry. Arrow indicates positive tubules. Scale bars = 100 μm. Data represent one of at least three technical replication each.
