Fig. 1: Overview and evaluation of vRIC-seq technology.
From: The architecture of the SARS-CoV-2 RNA genome inside virion
a Scheme of vRIC-seq technology. Concanavalin A (ConA) beads were used to capture the virion for diverse enzyme treatments in subsequent steps. b The proportions of chimeric reads mapped to SARS-CoV-2. c Scatter plots showing the correlation between two biological replicates for the number of chimeric reads (interaction strength). R, Pearson correlation coefficient. d Circos plot showing the distribution of chimeric reads along the SARS-CoV-2 genome. The inner red circle stands for the fractions of adenine or uracil within 100 nt windows, and the outer blue circle shows the coverage of chimeric reads. FSE frame-shifting element. e, f vRIC-seq confirmed known coronavirus RNA structures in the 5′ UTR (1–480 nt, e) and 3′ UTR (29,546–29,870 nt, f) of the SARS-CoV-2 RNA genome. Connection scores shown in different colors were used for assessing the base-pairing probability. The dashed lines illustrated the pseudoknot.
